Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549) (2024)

Mortaz, E., Alipoor, S. D., Movassaghi, M., Varahram, M., Ghorbani, J., Folkerts, G., Garssen, J., & Adco*ck, I. M. (2017). Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). BMC Pulmonary Medicine, 17(1), Article 68. https://doi.org/10.1186/s12890-017-0413-7

Mortaz, Esmaeil ; Alipoor, Shamila D. ; Movassaghi, Masoud et al. / Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). In: BMC Pulmonary Medicine. 2017 ; Vol. 17, No. 1.

@article{4fe5a8eb992745018eea81f001bd5b1a,

title = "Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)",

abstract = "Background: Tuberculosis (TB) is a major global health problem, and there is an association between tobacco smoke and TB. Water pipe smoking has become an increasing problem not only in Middle Eastern countries but also globally because users consider it as safer than cigarettes. The presence of high levels of toxic substances in water-pipe smoke may be a predisposing factor that enhances the incidence of pulmonary disorders. For example, uncontrolled macropinocytosis in alveolar epithelial cells following exposure to water-pipe smoke may predispose subjects to pulmonary infection. Here, we studied the effects of water-pipe condense (WPC) on the internalization of Mycobacterium Bovis BCG by macropinocytosis in the alveolar epithelial cell line A549. Methods: A549 cells were exposed to WPC (4 mg/ml) for 24, 48, 72 and 96 h. Cell viability was studied using the methyl thiazolyldipenyl-tetrazolium bromide (MTT) reduction assay and proliferation by bromodeoxyUridine (BrdU) incorporation. Cells were exposed to FITC-Dextran (1 mg/ml) (as a control) and FITC-BCG (MOI = 10) for 20 min at 37 °C before cells were collected and the uptake of BCG-FITC determined by flow cytometry. Similar experiments were performed at 4 °C as a control. The Rho-associated protein kinase (ROCK) inhibitor Y-27632 (1 μM) was used to assess the mechanism by which WPC enhanced BCG uptake. Results: WPC (4 mg/ml) increased the uptake of BCG-FITC after 72 (1.3±0.1 fold, p",

keywords = "Endocytosis Activity, Type II Alveolar Epithelial Cells (A549), Water pipe, 4 (1 aminoethyl) n (4 pyridyl)cyclohexanecarboxamide, cigarette smoke condensate, fluorescein isothiocyanate dextran, Rho kinase, A-549 cell line, article, BrdU assay, cell proliferation, cell viability, concentration (parameters), controlled study, flow cytometry, human, human cell, MTT assay, Mycobacterium bovis BCG, nonhuman, pinocytosis, temperature",

author = "Esmaeil Mortaz and Alipoor, {Shamila D.} and Masoud Movassaghi and Mohammad Varahram and Jahangir Ghorbani and Gert Folkerts and Johan Garssen and Adco*ck, {Ian M.}",

year = "2017",

month = apr,

day = "21",

doi = "10.1186/s12890-017-0413-7",

language = "English",

volume = "17",

journal = "BMC Pulmonary Medicine",

issn = "1471-2466",

publisher = "BioMed Central",

number = "1",

}

Mortaz, E, Alipoor, SD, Movassaghi, M, Varahram, M, Ghorbani, J, Folkerts, G, Garssen, J & Adco*ck, IM 2017, 'Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)', BMC Pulmonary Medicine, vol. 17, no. 1, 68. https://doi.org/10.1186/s12890-017-0413-7

Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). / Mortaz, Esmaeil; Alipoor, Shamila D.; Movassaghi, Masoud et al.
In: BMC Pulmonary Medicine, Vol. 17, No. 1, 68, 21.04.2017.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549)

AU - Mortaz, Esmaeil

AU - Alipoor, Shamila D.

AU - Movassaghi, Masoud

AU - Varahram, Mohammad

AU - Ghorbani, Jahangir

AU - Folkerts, Gert

AU - Garssen, Johan

AU - Adco*ck, Ian M.

PY - 2017/4/21

Y1 - 2017/4/21

N2 - Background: Tuberculosis (TB) is a major global health problem, and there is an association between tobacco smoke and TB. Water pipe smoking has become an increasing problem not only in Middle Eastern countries but also globally because users consider it as safer than cigarettes. The presence of high levels of toxic substances in water-pipe smoke may be a predisposing factor that enhances the incidence of pulmonary disorders. For example, uncontrolled macropinocytosis in alveolar epithelial cells following exposure to water-pipe smoke may predispose subjects to pulmonary infection. Here, we studied the effects of water-pipe condense (WPC) on the internalization of Mycobacterium Bovis BCG by macropinocytosis in the alveolar epithelial cell line A549. Methods: A549 cells were exposed to WPC (4 mg/ml) for 24, 48, 72 and 96 h. Cell viability was studied using the methyl thiazolyldipenyl-tetrazolium bromide (MTT) reduction assay and proliferation by bromodeoxyUridine (BrdU) incorporation. Cells were exposed to FITC-Dextran (1 mg/ml) (as a control) and FITC-BCG (MOI = 10) for 20 min at 37 °C before cells were collected and the uptake of BCG-FITC determined by flow cytometry. Similar experiments were performed at 4 °C as a control. The Rho-associated protein kinase (ROCK) inhibitor Y-27632 (1 μM) was used to assess the mechanism by which WPC enhanced BCG uptake. Results: WPC (4 mg/ml) increased the uptake of BCG-FITC after 72 (1.3±0.1 fold, p

AB - Background: Tuberculosis (TB) is a major global health problem, and there is an association between tobacco smoke and TB. Water pipe smoking has become an increasing problem not only in Middle Eastern countries but also globally because users consider it as safer than cigarettes. The presence of high levels of toxic substances in water-pipe smoke may be a predisposing factor that enhances the incidence of pulmonary disorders. For example, uncontrolled macropinocytosis in alveolar epithelial cells following exposure to water-pipe smoke may predispose subjects to pulmonary infection. Here, we studied the effects of water-pipe condense (WPC) on the internalization of Mycobacterium Bovis BCG by macropinocytosis in the alveolar epithelial cell line A549. Methods: A549 cells were exposed to WPC (4 mg/ml) for 24, 48, 72 and 96 h. Cell viability was studied using the methyl thiazolyldipenyl-tetrazolium bromide (MTT) reduction assay and proliferation by bromodeoxyUridine (BrdU) incorporation. Cells were exposed to FITC-Dextran (1 mg/ml) (as a control) and FITC-BCG (MOI = 10) for 20 min at 37 °C before cells were collected and the uptake of BCG-FITC determined by flow cytometry. Similar experiments were performed at 4 °C as a control. The Rho-associated protein kinase (ROCK) inhibitor Y-27632 (1 μM) was used to assess the mechanism by which WPC enhanced BCG uptake. Results: WPC (4 mg/ml) increased the uptake of BCG-FITC after 72 (1.3±0.1 fold, p

KW - Endocytosis Activity

KW - Type II Alveolar Epithelial Cells (A549)

KW - Water pipe

KW - 4 (1 aminoethyl) n (4 pyridyl)cyclohexanecarboxamide

KW - cigarette smoke condensate

KW - fluorescein isothiocyanate dextran

KW - Rho kinase

KW - A-549 cell line

KW - article

KW - BrdU assay

KW - cell proliferation

KW - cell viability

KW - concentration (parameters)

KW - controlled study

KW - flow cytometry

KW - human

KW - human cell

KW - MTT assay

KW - Mycobacterium bovis BCG

KW - nonhuman

KW - pinocytosis

KW - temperature

U2 - 10.1186/s12890-017-0413-7

DO - 10.1186/s12890-017-0413-7

M3 - Article

SN - 1471-2466

VL - 17

JO - BMC Pulmonary Medicine

JF - BMC Pulmonary Medicine

IS - 1

M1 - 68

ER -

Mortaz E, Alipoor SD, Movassaghi M, Varahram M, Ghorbani J, Folkerts G et al. Water-pipe smoke condensate increases the internalization of Mycobacterium Bovis of type II alveolar epithelial cells (A549). BMC Pulmonary Medicine. 2017 Apr 21;17(1):68. doi: 10.1186/s12890-017-0413-7